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Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation

Xiaomu Liu
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China
Abstract :
The study suggests that Sirtuin 1 (SIRT1) may play an important role in the development of bovine
adipose tissue
in vivo
. To investigate the differential mRNA expression between bovine intramuscular
(IM) preadipocytes (BIPs) and subcutaneous (SC) preadipocytes (BSPs), conventional collagenase-
based procedures were modified to harvest preadipocytes from
longissimus dorsi
muscle and
SC adipose tissue of Luxi cattle, respectively. The time-spatial expression of SIRT1 and associated
genes, such as forkhead box O1(FoxO1), peroxisome proliferator-activated receptor-γ (PPARγ),
insulin-like growth factor-1(IGF-1), glyceraldehydes-3-phosphate dehydrogenase(GAPDH), and
leptin, during bovine preadipocyte differentiation were investigated using real-time quantitative
PCR. The intracellular triglyceride concentration of BSPs increased more dramatically than that of
BIPs during cell differentiation. The expression of SIRT1 and other genes occurred in the BSPs and
BIPs during differentiation indicating that different mechanisms underlie the adipogenesis of BSPs
and BIPs, and that the BSPs cultured
in vitro
show a higher differentiation capacity than BIPs.
Keywords :
Bovine preadipocyte differentiation / FoxO1 / GAPDH / IGF-1 / leptin / PPARĪ³ / SIRT1

Date Deposited : 01 Apr 2015 15:26

Last Modified : 01 Apr 2015 15:26

Official URL:

Volume 30, Number 2, - 2012

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