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Animal Science Papers and Reports

A novel pig gene, CPNE1 , differentially expressed in the muscle tissues from Wujin pigs and Large White pigs

Pu Shifei
Department of Animal Husbandry and Veterinary, Yunnan Vocational and Technical College of Agriculture, Kunming 650212, China
Abstract :
The mRNA differential display technique was performed to investigate gene expression differences
in the
longissimus dorsi
muscle from Wujin and Large White pigs. A fragment of one differentially
expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained
using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene
encodes a protein of 537 amino acids, which is homologous with the Copine I (
CPNE1
) of eight
species: greater horseshoe bat (95%), mouse (92% ), rat (93%), cattle (95%), sheep (95%), rabbit
(94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the
swine
CPNE1
gene and had been assigned GeneID: 100233174. The phylogenetic analysis revealed
that the swine
CPNE1
gene has a closer genetic relationship with the
CPNE1
gene of a dog. The
tissue expression analysis indicated that the swine
CPNE1
gene has a broad tissue distribution. The
experiment described is the first to establish the primary foundation for further research on the
swine
CPNE1
gene.
Keywords :
PN1 / differential display / muscle / mRNA / pig

Date Deposited : 01 Apr 2015 15:27

Last Modified : 01 Apr 2015 15:27

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 2, - 2012

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