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.: Home > Animal Science Papers and Reports > 2012 > Volume 30 Number 2 > Jianliang Wu, Jianhua Liu, Yanan Yuan, Liying Qiao, Haibo Hao, Wenzhong Liu

Polymorphism of the beta 3-adrenergic receptor gene (ADRB3 ) and its distribution in domestic and wild pigs in China

Jianliang Wu, Jianhua Liu, Yanan Yuan, Liying Qiao, Haibo Hao, Wenzhong Liu
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
Abstract :
The beta 3-adrenergic receptor (ADRB3) is a G-protein coupled receptor encoded by
ADRB3
gene
and involved in regulation of lipolysis and homeostasis. The objective of this study was to detect the
genetic variation existed in this gene in eight domestic and wild pig populations. The polymorphism
of partial sequences of
ADRB3
gene in 336 pigs was analysed by single strand conformation
polymorphism technique based on PCR (PCR-SSCP).
Fst
statistic and analysis of molecular
variance (AMOVA) were used to measure the genetic differentiation. The genetic distances between
populations were computed and a phylogenic tree was reconstructed by the unweighted pair group
method using the arithmetic mean. Five haplotypes named A, B, C, D and E were detected with
the frequencies ranging from 0.060 for E to 0.324
for A. The reliability of all haplotype frequencies
reached 0.95. The haplotypes involved four polymorphic sites, of which one located in exon 2 with a
thymine insertion, and the remaining three in non-coding regions with G/A and G/T substitutions,
respectively. The thymine insertion resulted in the absence of two amino acids in the sequence
of receptor protein. The haplotype diversity ranged from 0.579±0.057 in pure wild Liaoning to
0.792±0.024 in Mashen pigs, indicating abundant genetic diversity to exist
in the populations. The
Fst
estimates showed that the degree of population differentiation between domestic and wild
pigs was moderate, but at a lower level within domestic populations. About 94.38% of the genetic
variation originated from within the populations by
AMOVA.
Keywords :
drenergic receptor / domestic pigs / wild pigs / genetic diversity / PCR-SSCP

Date Deposited : 01 Apr 2015 15:29

Last Modified : 01 Apr 2015 15:29

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 2, - 2012

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