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Animal Science Papers and Reports

Adaptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene

Grzegorz Smołucha
Department of Animal Cytogenetics and Molecular Genetics of the National Research Institute for Animal Production, Krakowska 1, 32-083 Balice / Cracow, Poland
Abstract :
The paper describes adaptation of primer extension method for genotyping scrapie-associated
codons 136, 141 and 156 in sheep
PRNP
gene. As the method failed to genotype the polymorphism
in codon 171 in some samples, this polymorphism was excluded from the assay. Such genotyping
problems were not observed in earlier studies describing the method and cannot be easily explained.
However, full accordance of genotypes in codons 136, 141 and 156 with sequencing results was
obtained and thus the method can be recommended for routine application. Simultaneously, we
strongly recommend to pay special attention when using the minisequencing method for genotyping
codon 171 in sheep
PRNP
.
Keywords :
RNP / scrapie / sheep /SNaPshot

Date Deposited : 01 Apr 2015 15:35

Last Modified : 01 Apr 2015 15:35

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 2, - 2012

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