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Animal Science Papers and Reports

Effect of DGAT1 variants on milk composition traits in Iranian Holstein cattle population

Hamed Kharrati Koopaei
Department of Animal Science, Shahid Bahonar University of Kerman, Iran
Abstract :
The results from a number of QTL mapping studies recently provided the evidence for quantitative
trait
loci
affecting milk yield and milk fat content localized on bovine chromosome 14 (BTA14)
close to
DGAT1
gene.
DGAT1
gene codes for diacylglycerol-acyltransferase enzyme which plays a
main role in the triglyceride synthesis and subsequently the milk composition. A transition mutation
in this gene results in substitution of guanine by adenine leading to the substitution of lysine by
alanine in diacylglycerol-acyltransferase enzyme. In this study, 398 blood samples were collected
from the Holstein dairy farms in Iran. A 411 bp fragment of exon 8 in
DGAT1
gene was amplified
and the animals were genotyped using RFLP-PCR technique. Three genotypes including KK (36
animals), AA (136 animals) and KA (226 animals) were identified. Frequencies of K and A (mutant
type) alleles were estimated to be 0.37 and 0.63, respectively. The effect of
DGAT1
gene on FAT
2x
(milk fat yield adjusted for two milkings per day, kg), FAT
P2X
(fat content of milk adjusted for two
milkings per day, %), EBV
FP
(estimated breeding value for fat content of milk, %) and PRO
PER305
(milk protein content adjusted for 305 days, %)
occurred highly significant (P<0.001)
Keywords :
dairy cattle / DGAT1 gene / fat / milk composition / PCR-RFLP /protein

Date Deposited : 01 Apr 2015 15:45

Last Modified : 01 Apr 2015 15:45

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 3, - 2012

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