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.: Home > Animal Science Papers and Reports > 2012 > Volume 30 Number 3 > X.G. Yang, X.W. Wang, E.W. Zuo, C.L Hao, S.S. Lu, H.B. Liu, H.Y. Xu, Y.Q. Lu, K.H. Lu

cDNA cloning of porcine CDH1 and its expression profile in porcine early parthenotes

X.G. Yang, X.W. Wang, E.W. Zuo, C.L Hao, S.S. Lu, H.B. Liu, H.Y. Xu, Y.Q. Lu, K.H. Lu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi 530004, China
Abstract :
The study aimed at cloning the complete cDNA sequence of porcine CDH1 gene and detecting its
expression in different developmental stages of early parthenotes. After cloning the CDH1 gene
from porcine oviduct using rapid amplification of cDNA ends (RACE) method, the sequence
analysis revealed that porcine CDH1 gene complete cDNA nucleotide sequence amounted to 4283
bp including 2652 bp of open reading frame (ORF), 105 bp of 5’ untranslated region (UTR) and
1526 bp of 3’ UTR. The ORF encoded a deduced protein precursor of 97 kD with 883 amino acid
residues. The precursor protein including signal peptide, extracellular region, membrane-spanning
region and cytoplasmic region had a single transmembrane structure, and its extracellular region
had HAV motif and some Ca
2+
binding regions. The porcine CDH1 protein showed high homology
with cattle (89%), horse (87%), dog (86%), human (84%), chimpanzee (83%) and mouse (83%)
CDH1. The results from RT-PCR and real-time PCR indicated that CDH1 gene could be expressed
in both immature and mature oocytes, as well as early parthenotes (2-, 4-, 8-cell embryos, morula,
blastocysts). The data of real-time PCR showed that the expression of CDH1 was the highest at 2-
cell parthenogenetic embryos stage, and then it decreased significantly till 8-cell embryos stage; the
second increased expression level occurred at morulas stage, and then also showed a diminishing
trend in the following developmental stage. This is the first report of cloning and analysing the
porcine CDH1 cDNA that provides critical information for further research of its functions in pig
embryo development.
Keywords :
CDH1 / gene cloning / gene expression /porcine parthenotes

Date Deposited : 01 Apr 2015 15:46

Last Modified : 01 Apr 2015 15:46

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 3, - 2012

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