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.: Home > Animal Science Papers and Reports > 2012 > Volume 30 Number 4 > Marcin Samiec**, Maria Skrzyszowska

High developmental capability of porcine cloned embryos following trichostatin A-dependent epigenomic transformation during in vitro maturation of oocytes pre-exposed to R -roscovitine

Marcin Samiec**, Maria Skrzyszowska
Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice/Kraków, Poland
Abstract :
The study was carried out in order to evolve, adopt and optimize the new system for preparation
of nuclear recipient cells at different stages preceding the somatic cell nuclear transfer (SCNT)
in pigs, including
in vitro
maturation (IVM) of oocytes. The system was applied to facilitate and
accelerate the epigenomic reprogrammability for gene expression of donor cell nuclei that had been
transplanted into cytoplasmic microenvironment of recipient oocytes and subsequently underwent
the dedifferentiating and re-establishing the totipotent epigenetically conditioned status of their
transcriptional activity during the preimplantation development of cloned embryos. The use of
trichostatin A (TSA)-mediated epigenetic modulation of
in vitro
-maturing porcine nuclear recipient
oocytes that had been pre-treated with
R
-roscovitine (
R-
RSCV) resulted in significantly increased
blastocyst formation rate among the cloned embryos compared to the
R-
RSCV- and TSA-unexposed
group (almost 44%
vs.
26%).
Keywords :
pig / oocyte / in vitro maturation / R -roscovitine / trichostatin A / epigenomic maturity / somatic cell nuclear transfer / cloned embryo / epigenetic reprogramming

Date Deposited : 01 Apr 2015 16:11

Last Modified : 01 Apr 2015 16:11

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34&o=7497

Volume 30, Number 4, - 2012

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