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Animal Science Papers and Reports

Effects of vitrification of partially denuded bovine immature oocytes

Krzysztof Papis
Department of Experimental Embryology, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzębiec, 05-552 Magdalenka, Poland
Abstract :
In spite of many trials on bovine oocyte cryopreservation, their efficiency remains unsatisfactory,
regardless of method used. Although a births of about 15 calves obtained after transfer of embryos
developed from frozen or vitrified oocytes were reported by now, a total efficiency of oocyte
cryopreservation, in terms of blastocyst rate obtained, reaches approximately a half of those
reported for fresh oocytes. The authors’ former research on mature bovine oocyte vitrification
showed a possibility of obtaining a very high oocyte survival rate and development of up to 29.6%
of blastocysts. In the present paper, reported and discussed are results of similar vitrification
procedure, but employed for germinal vesicle stage (GV) of bovine oocytes. Immature cumulus-
oocyte complexes (COCs) obtained from ovarian follicles were vortexed for short time to strip off a
portion of cumulus cells. Vitrification was performed using microdroplet method in VS14 solution,
after 12-15 min pre-equilibration and 30 or 45 s equilibration periods. Warming was accomplished
in warm dilution medium, without sucrose. After
in vitro
maturation and fertilization, presumptive
zygotes were cultured
in vitro
up to day 10. Depending on pre-equilibration parameters 5.3- 8.4%
blastocysts developed from vitrified oocytes, that is significantly less compared to blastocyst ratio
obtained from control, fresh oocytes (40.9%, P≤0.01, Fisher test). Although the ratio of blastocysts
obtained from vitrified GV oocytes presented in this paper does not differ from the best results
reported elsewhere, the autors suggest a need of further extensive research on bovine immature
oocyte vitrification to obtain replicable, satisfactory efficiency.
Keywords :
blastocyst /cattle / immature oocytes / pre-equilibration / vitrification

Date Deposited : 02 Apr 2015 09:34

Last Modified : 02 Apr 2015 09:34

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 31, Number 1, - 2013

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