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Polymorphism of AA-NAT gene and its relationship with litter size of Jining Grey goat of China

Mingxing CHU
Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Abstract :
The polymorphisms of arylalkylamine-N-acetyltransferase (AA-NAT) gene in high-prolificacy
Jining Grey goat, medium-prolificacy Boer goat and low-prolificacy Liaoning Cashmere, Inner
Mongolia Cashmere and Angora goats were detected to analyse their relationships with litter size.
Primers (P1-P5) were designed to detect the polymorphisms by PCR-SSCP and PCR-RF
l
P. For P2,
AA, AB
and
BB
genotypes were detected. Sequencing revealed one silent mutation (T132C) of
AA-
NAT
gene in
BB
in comparison to
AA.
For P3,
CC
and
CD
genotypes were detected and sequencing
revealed one mutation (C265T) of
AA-NAT
gene in
CD
in comparison to
CC
, and this mutation
resulted in an amino acid change of Arg→Cys (R89C). The Jining Grey does with genotype
CD
delivered by 0.56 kids (
P
<
0.05) more than those with
CC
genotype. For P5,
EE
,
EF
and
FF
genotypes
were detected and sequencing revealed one mutation (C586T) of
AA-NAT
gene in
FF
in comparison
16
to
EE
. This mutation caused an amino acid change of Arg→Trp (R196W). For both P2 and P5, the
differences in litter size among three genotypes were not significant in Jining Grey goats (
P
>0.05).
These results preliminarily indicate that allele D at the C265T
locus
of
AA-NAT
gene is a potential
marker in genetic improvement of litter size in goats.
Keywords :
16 to EE . This mutation caused an amino acid change of Arg→Trp (R196W). For both P2 and P5, the differences in litter size among three genotypes were not significant in Jining Grey goats ( P >0.05). These results preliminarily indicate that allele D at the C265T locus of AA-NAT gene is a potential marker in genetic improvement of litter size in goats. K e Y WORDS:

Date Deposited : 02 Apr 2015 09:36

Last Modified : 02 Apr 2015 09:36

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 31, Number 1, - 2015

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