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Animal Science Papers and Reports

HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds

Hai-chao Lin
College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
Abstract :
Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different
breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods.
Three Huai and three Landrace pigs each were individually housed at the Fujian Shanghang Huai
pig breeding farm under similar feeding conditions. Pigs were humanely slaughtered at saleable
weight and samples of kidney, liver and skeletal muscle were collected for expression analyses.
HMGR
mRNA expressions in liver, muscle and kidney of Huai pigs both occurred higher than
expression found in the Landrace. Positive staining of HMGR protein was revealed in the three
tissues considered. The HMGR protein expression level was not significant statistically in liver,
muscle and kidney across two breeds, but the amount of Huai pig HMGR protein expressed in
three tissues was higher than that of the Landrace. The level of HMGR protein expression in liver
was higher than in the muscle and renal tissues (
P<
0.05). The results demonstrate that
HMGR
expression in pigs may depend on tissue and species.
Keywords :
HMGR / immunohistochemistry / RT-PCR / pig

Date Deposited : 02 Apr 2015 09:38

Last Modified : 02 Apr 2015 09:38

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 31, Number 1, - 2013

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