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Animal Science Papers and Reports

Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran

Rasoul Khatami Nejhad
Department of Animal Sciences, Faculty of Agriculture, Gonbad University, P.O. Box 4971799151, Golestan, Gonbad, Iran
Abstract :
To study the genetic polymorphism of κ-casein (
CSN3
) gene as candidate gene for marker-assisted
selection in different
domestic livestock species, blood samples were randomly withdrawn from
34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment
from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products
were analysed using single strand conformation polymorphism (SSCP) method. Results indicated
that there were two (K
1
and K
2
) conformational patterns with frequencies 0.91 and 0.09 for Alpine
and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg
equilibrium for the gene in both breeds.
Keywords :
CSN3 / goat / genetic polymorphism / SSCP

Date Deposited : 02 Apr 2015 09:49

Last Modified : 02 Apr 2015 09:49

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 31, Number 2, - 2013

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