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The use of encapsulated Ovalbumin-LHRH-7 (OL) protein in single-dose vaccination protocols for LHRH immunization

Hasan Ülker
Yüzüncü Yil University Faculty of Agriculture Department of Animal Sciences, 65080 Van, Turkey
Abstract :
The objective of this study was to evaluate effectiveness of Ovalbumin-LHRH-7 (OL) protein when
injected in crude, purified, free or encapsulated forms and using a single vaccination protocol along
with CpG, inulin and saponin adjuvants. Fifty six C57BL/6 mice in seven groups (n=8) received
various treatments and doses: Group 1 was control; Group 2 and 3 were injected twice with purified
or crude OL protein, respectively, 4 wks apart. Group 4 and 5 were injected only once with purified
or crude OL protein, respectively. Group 6 was injected only once with a mix of purified OL protein
and encapsulated purified OL protein. Group 7 was injected only once with a mix of crude OL protein
and encapsulated crude OL protein. There was an immunization effect observed on the I
binding (P<0.05). Antibodies (Abs) against LHRH were identified on week 5 of immunization in groups
2, 3 and 4. Boosting at week 5 caused a significant increase in LHRH antibody (Ab) concentrations in
groups 2 and 3. Numbers of pregnant animals and prengnancy rates were suppressed in all treatment
groups at various degrees (P<0.05). Numbers of pups born were affected by immunization (P<0.05).
Concluding, immunization with OL protein generated either biological or both immunological and
biological effects in the most of treatment groups. The study confirmed the earlier findings that
purified OL protein with CpG adjuvant is effective in inducing immune response and suppressing
reproductive functions. However, the original idea that the non-capsulated antigen/adjuvant mix
would work as primary injection, while encapsulated counterpart would mimic booster injections in a
single vaccination protocol could not be confirmed in this study. Further studies to determine affecting
factors for single-injection LHRH immunization are needed.
Keywords :
encapsulation / immunization / LHRH / mice / reproduction

Date Deposited : 02 Apr 2015 10:11

Last Modified : 02 Apr 2015 10:11

Official URL:

Volume 31, Number 3, - 2013

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