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Expression of PC , PCK1 , PCK2 , LDHB , FBP1 and G6PC genes in the liver of cows in the transition from pregnancy to lactation

Małgorzata Ostrowska
Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland
Abstract :
The effect of the transition of dairy cows from pregnancy to lactation on the expression of genes
encoding enzymes involved in hepatic glucose metabolism was studied. Six Holstein-Friesian
heifers were used for this study. Liver samples were collected by biopsy on day 7 before expected
parturition (-7) and days 3 and 21 after parturition (+3 and +21, respectively). The mRNA levels of
pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase 1 (PCK1), phosphoenolpyruvate
carboxykinase 2 (PCK2), lactate dehydrogenase B (LDHB), fructose-1,6-bisphosphatase 1 (FBP1)
and glucose-6-phosphatase (G6PC) were measured using quantitative real-time PCR. The expression
of PC and PCK2 mRNA on day 3 of lactation was significantly higher than that on day 7 before
parturition (P<0.05) and slightly higher than on day 21 postpartum. The
gene showed the
highest expression level on day 3 of lactation, as compared with day 7 prepartum (P<0.001) and day
21 postpartum (P<0.001). No differences were shown in
expression levels

between pregnancy and early lactation

Keywords :
airy cow / gene expression / glucose metabolism / liver

Date Deposited : 02 Apr 2015 10:13

Last Modified : 02 Apr 2015 10:13

Official URL:

Volume 31, Number 4, - 2013

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