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Animal Science Papers and Reports

Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep

Feng Guan
College of Life Sciences, China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China
Abstract :
The FecB gene has been shown to be crucial in reproduction in many sheep breeds. It is a single
nucleotide polymorphism (SNP) located in the bone morphogenetic protein receptor IB (BMPR-
IB) gene. The current methods for genotyping the FecB mutation are either slow and laborious
or expensive. In this report, a single-step amplification approach suitable for FecB genotyping
method is described. Multiplex PCR was performed with four primers on the basis of tetra-
primer amplification refractory mutation system PCR (tetra-primer ARMS PCR), then three FecB
genotypes can be detected after electrophoresis. Genotyping results of the proposed multiplex PCR
occurred to be in complete accordance with forced PCR-RFLP of all samples. It is a rapid and
simple method for detection of FecB in a larger number of samples, and is suitable for other SNPs
detection with specific primers even in most “low-tech” laboratories.
Keywords :
rapid detection / sheep FecB gene / tetra-ARMS PCR

Date Deposited : 02 Apr 2015 13:32

Last Modified : 02 Apr 2015 13:32

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 32, Number 3, - 2014

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