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.: Home > Animal Science Papers and Reports > 2014 > Volume 32 Number 4 > Marzena Mogielnicka-Brzozowska**, Jerzy Strzeżek, Anna Dziekońska, Władysław Kordan

The ability of zinc-binding proteins from boar seminal plasma to bind heparin and phosphorylcholine

Marzena Mogielnicka-Brzozowska**, Jerzy Strzeżek, Anna Dziekońska, Władysław Kordan
University of Warmia and Mazury in Olsztyn, Faculty of Animal Bioengineering, Department of Animal Biochemistry and Biotechnology, Oczapowskiego 5, 10-718 Olsztyn, Poland
Abstract :
Zinc-binding proteins (ZnBPs) isolated from boar seminal plasma exert multiple effects on boar
spermatozoa. The aim of this study was to determine whether the isolated zinc-binding proteins were
capable of binding
additional ligands. The affinity chromatography method was used for the first
time to demonstrate that boar ZnBPs show affinity for both heparin (hep) and phosphorylcholine
(pch). ZnBPs
hep accounted for approximately 20%, and ZnBPs
pch – for approximately 45% of
total ZnBPs. Predominant polypeptides with molecular mass form 6.5 to 14 kDa were observed in
both groups (ZnBPs
hep, ZnBPs
pch). The analyzed peptides’ low molecular mass and their ability
to bind zinc ions as well as heparin and phosphorylcholine suggest that they belong to the family of
multifunctional boar spermadhesins which perform their functions in the fertilization process by
binding more than one ligand. The results indicate that the ZnBPs of boar seminal plasma may be
involved in the processes associated with oocyte fertilization not only by zinc ions binding but also
through heparin and phosphorylcholine.
Keywords :
boar seminal plasma / heparin-binding protein / phosphorylcholine-binding protein / zinc-binding proteins

Date Deposited : 02 Apr 2015 13:48

Last Modified : 02 Apr 2015 13:48

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Volume 32, Number 4, - 2014

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