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.: Home > Animal Science Papers and Reports > 2015 > Volume 33 Number 1 > Ewa Osińska, Małgorzata Gajewska, Alicja Majewska, Tomasz Motyl

Quantification of bovine mammary stem/progenitor cells by laser scanning and flow cytometry

Ewa Osińska, Małgorzata Gajewska, Alicja Majewska, Tomasz Motyl
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland
Abstract :
Due to the presence and activity of mammary stem cells (MaSC), growth and remodelling of
mammary gland during puberty and lactation cycles is possible. In this study the number of putative
mammary stem/progenitor cells was examined in 20 months old non-pregnant Holstein-Friesian
heifers. Cells were double-stained with fluorescent dye-conjugated antibodies against stem cell
antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B), and were analysed using
scanning cytometry and flow cytometry. Nuclei were counterstained with Hoechst 33342. Scanning
and flow cytometry revealed 2.43±0.32% and 1±0.37% of MaSC in total cell number, respectively.
Sca-1
pos
FNDC3B
pos
cells did not express estrogen receptor (ER
α
), confirming their undifferentiated
phenotype. In conclusion, scanning cytometry is a preferable method for evaluation of the number
and localization of MaSC
in situ
, whereas, flow cytometry with cell sorting enables further genomic
and biochemical analyses of isolated cells.
Keywords :
flow cytometry / mammary gland / progenitor cells / scanning cytometry / stem

Date Deposited : 02 Apr 2015 13:55

Last Modified : 02 Apr 2015 13:55

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 33, Number 1, - 2015

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