UMM Logo

Universitas Muhammadiyah Malang

Free Download Journals Directory

Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2013 > Volume 62 Number 1 > Zorica JOVANOVIC, Svetlana JOVANOVIC

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech Haemopis sanguisuga

Department of Pathological Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
Abstract :

Oxidative stress and the production of reactive oxygen species are known to play a major role in neuronal cell damage, but the exact mechanisms responsible for neuronal injury and death remain uncertain. In the present study, we examined the effects of oxidative stress on spontaneous spike activity and depolarizing outward potassium current by exposing the Retzius neurons of the leech to cumene hydroperoxide (CHP) and hydrogen peroxide (H2O2), the oxidants commonly used to examine oxidative mechanisms mediating cell death. We observed that relatively low concentrations of CHP (0.25, 1, and 1.5 mM) led to a marked prolongation of spontaneous repetitive activity. The prolonged action potentials showed an initial, spike-like depolarization followed by a plateau phase. In contrast, H2O2 at the same and much higher concentrations (0.25 to 5 mM) did not significantly change the duration of spontaneous spike potentials of leech Retzius nerve cells (LRNCs). In the voltage clamp experiments, calcium-activated outward potassium currents, needed for the repolarization of the action potential, were suppressed with CHP, but not with H2O2. The present findings indicate that CHP is a more potent oxidant and neurotoxin than H2O2 and that the effect of CHP on the electrophysiological properties of LRNCs may be due to the inhibition of the potassium channels.

Keywords :
cumene hydroperoxide, hydrogen peroxide, leech, oxidative stress, potassium currents

Date Deposited : 06 Apr 2015 11:06

Last Modified : 06 Apr 2015 11:06

Official URL: http://

Volume 62, Number 1, January 2013

Full Text Original
Abstract : pdf doc