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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2013 > Volume 62 Number 1 > Nobuhito HAYASHIMOTO, Hanako MORITA, Tomoko ISHIDA, Masahiko YASUDA, Shuko KAMEDA, Ritsuki UCHIDA, Mai TANAKA, Midori OZAWA, Azusa SATO, Akira TAKAKURA, Toshio ITOH, Naoko KAGIYAMA

Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan

Nobuhito HAYASHIMOTO, Hanako MORITA, Tomoko ISHIDA, Masahiko YASUDA, Shuko KAMEDA, Ritsuki UCHIDA, Mai TANAKA, Midori OZAWA, Azusa SATO, Akira TAKAKURA, Toshio ITOH, Naoko KAGIYAMA
ICLAS Monitoring Center, Central Institute for Experimental Animals, 3–25–12 Tonomachi, Kawasaki, Kawasaki, Kanagawa 210-0821, Japan
Abstract :

On the basis of our 2011 microbiological monitoring tests, we report here the current microbiological status of mice and rats housed in experimental facilities in Japan. We tested more than 14,000 mice, 6,000 serum samples, 500 fecal or cecal samples, and 200 lung samples from 3,549 mouse facilities within Japanese universities and institutes (U/I), pharmaceutical companies and contract research organizations (P/C). We also tested more than 1,500 rats, 1,600 serum samples, and 20 fecal or cecal samples from 772 U/I and P/C rat facilities. Bacterial cultures, serology, microscopy, PCR, and DNA analysis using DNA chips were performed. Staphylococcus aureus (18.8% in mouse facilities, 58.6% in rat facilities) was the most prevalent agent in both the mouse and rat facilities. The next most prevalent agents in the mouse facilities were murine norovirus (11.97%), intestinal protozoa (0.05-8.49%, from various species), Pasteurella pneumotropica (5.32%), and Helicobacter hepaticus (3.17%), while intestinal protozoa (0.74-6.84% from various species), Syphacia muris (6.20%), Pseudomonas aeruginosa (3.61%), and Pasteurella pneumotropica (3.05%) were the subsequent most prevalent agents in the rat facilities. These results suggest that the currently prevalent microbes in laboratory mice and rats in Japan are mainly opportunistic pathogens, intestinal protozoa, and microbes with low pathogenicity.

Keywords :
mice, microbiological monitoring, rats

Date Deposited : 06 Apr 2015 11:14

Last Modified : 06 Apr 2015 11:14

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 62, Number 1, - 2013

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