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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2013 > Volume 62 Number 1 > Noriko HIRAIWA, Mutsumi ISHIMOTO, Hiroshi YASUE

Examination of the Mouse Embryo by Micro-CT

Noriko HIRAIWA, Mutsumi ISHIMOTO, Hiroshi YASUE
RIKEN BioResource Center, 3–1–1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan 2)MARS Tohken X-Ray Inspection Co. Ltd., 2–27–7 Tamagawa, Chofu, Tokyo 182-0025, Japan 3)Tsukuba Gene Technology Laboratories, 6–446 Arakawaoki, Tsuchiura, Ibaraki 300-0873, Japan
Abstract :

Micro X-ray computed tomography (micro-CT) is widely used in preclinical studies of small animals. However, due to the low soft tissue contrast, segmentation of soft tissues in the micro-CT image is a challenging problem. To gain a better understanding of the macroscopic anatomy of the mouse embryo, 3 fixation methods and 3 metal stainings were examined for micro-CT using C57BL/6J mouse embryos in the present study. The examination demonstrated that 1% acetic acid/95% ethanol fixative together with zinc staining provided a high contrast micro-CT image, enabling the segmentation of soft tissues. Then, using this condition, the macroscopic embryo structure of the nude mouse was examined, revealing lack of a thymus. It appears that micro-CT with the fixation and staining condition devised in the present study could be a powerful tool in detecting the effects of various mutations at embryonic stages.

Keywords :
embryo, nude mice, X-ray computed tomography

Date Deposited : 06 Apr 2015 11:19

Last Modified : 06 Apr 2015 11:19

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 62, Number 1, - 2013

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