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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2013 > Volume 62 Number 2 > Takao UKAJI, Hideyuki MATOBA, Hiroshi UCHIYAMA, Osamu KAI

Use of Genomic In Situ Hybridization to Identify Chromosomes in Gerbil-Mouse Heterohybridomas

Takao UKAJI, Hideyuki MATOBA, Hiroshi UCHIYAMA, Osamu KAI
Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
Abstract :

Heterohybridomas, created to secrete monoclonal antibodies, are generally unstable over long-term culture because of chromosome elimination during culture. To evaluate the karyotype of heterohybridomas, we used simultaneous genomic in situ hybridization (GISH), which can distinguish unambiguously between parental genomes in allopolyploid species. Using GISH, we discriminated gerbil and mouse chromosomes in heterohybridomas with high sensitivity. The GISH technique will allow evaluation of the stability and crossability of heterohybridomas made from the fusion of mouse cells with those of related species.

Keywords :
heterohybridoma, Mongolian gerbil, simultaneous genomic in situ hybridization (GISH)

Date Deposited : 06 Apr 2015 11:38

Last Modified : 06 Apr 2015 11:38

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 62, Number 2, - 2013

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