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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 1 > Kazuya Sakata, Masaki Ohmuraya, Kimi Araki, Chigure Suzuki, Satoshi Ida, Daisuke Hashimoto, Jung Wang, Yasuo Uchiyama, Hideo Baba, Ken-ichi Yamamura

Generation and Analysis of Serine Protease Inhibitor Kazal Type 3-Cre Driver Mice

Kazuya Sakata, Masaki Ohmuraya, Kimi Araki, Chigure Suzuki, Satoshi Ida, Daisuke Hashimoto, Jung Wang, Yasuo Uchiyama, Hideo Baba, Ken-ichi Yamamura
Institute of Resource Development and Analysis, Kumamoto University, 2–2–1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan
Abstract :

Serine protease inhibitor Kazal type 1 (SPINK1; mouse homologue Spink3) was initially discovered as a trypsin-specific inhibitor in the pancreas. However, previous studies have suggested that SPINK1/Spink3 is expressed in a wide range of normal tissues and tumors, although precise characterization of its gene expression has not been described in adulthood. To further analyze Spink3 expression, we generated two mouse lines in which either lacZ or Cre recombinase genes were inserted into the Spink3 locus by Cre-loxP technology. In Spink3lacZ mice, β-galactosidase activity was found in acinar cells of the pancreas and kidney, as well as epithelial cells of the bronchus in the lung, but not in the gastrointestinal tract or liver. Spink3cre knock-in mice were crossed with Rosa26 reporter (R26R) mice to monitor Spink3 promoter activity. In Spink3cre;R26R mice, β-galactosidase activity was found in acinar cells of the pancreas, kidney, lung, and a small proportion of cells in the gastrointestinal tract and liver. These data suggest that Spink3 is widely expressed in endoderm-derived tissues, and that Spink3cre knock-in mice are a useful tool for establishment of a conditional knockout mice to analyze Spink3 function not only in normal tissues, but also in tumors that express SPINK1/Spink3.

Keywords :
SPINK1, Spink3, Spink3 cre mice, Spink3 lacZ mice

Date Deposited : 06 Apr 2015 13:30

Last Modified : 06 Apr 2015 13:30

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 63, Number 1, - 2014

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