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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 2 > Mathurot Duangchanchot, Rapee Inpunkaew, Pravate Thongsiri, Nobuhito Hayashimoto, Nobuhiro Gemma, Masaru Nikaido, Masayoshi Takahashi, Kanchana Kengkoom

Prevalence of Helicobacter in Laboratory Micein Thailand

Mathurot Duangchanchot, Rapee Inpunkaew, Pravate Thongsiri, Nobuhito Hayashimoto, Nobuhiro Gemma, Masaru Nikaido, Masayoshi Takahashi, Kanchana Kengkoom
National Laboratory Center, Mahidol University, Salaya, Buddhamonthon, Nakhonprathom 73170, Thailand
Abstract :

Prevalence of Helicobacter is mostly unknown in laboratory animals in Thailand. The 221 mice feces/cecum from 8 universities, 2 pharmaceutical companies and 3 research institutions in Thailand were surveyed for the prevalence and distribution of Helicobacter species by using the Electrochemical DNA chip. Helicobacter were detected 23/46 samples in Specific Pathogen Free (SPF) and 168/175 in conventional condition. Prevalence of Helicobacter were 98%, 96%, 92% and 78% in South (n=40), Northeast (n=40), North (n=25) and Central area (n=116), respectively. Only Central area holds SPF facility resulting in Helicobacter prevalence that seems to be lower than other areas. Three species of Helicobacter were detected in feces/cecum samples by sequence analysis: H. rodentium (67.0%, 148 samples), Helicobacter sp. MIT 01-6451 (15.4%, 34 samples), and unidentified Helicobacter species (14.1%, 9 samples). The results suggested that H. rodentium is the most common species of Helicobacter in laboratory mice in Thailand.

Keywords :
DNA chip, Helicobacter , laboratory mice, prevalence

Date Deposited : 07 Apr 2015 10:02

Last Modified : 07 Apr 2015 10:02

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 63, Number 2, - 2014

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