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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 2 > Shunichi Suzuki, Misae Suzuki, Michiko Nakai, Shoichiro Sembon, Daiichiro Fuchimoto, Akira Onishi

Transcriptional and Histological Analyses of the Thymic Developmental Process in the Fetal Pig

Shunichi Suzuki, Misae Suzuki, Michiko Nakai, Shoichiro Sembon, Daiichiro Fuchimoto, Akira Onishi
Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan
Abstract :

The humanized pig model, in which human cells or tissues can be functionally maintained in pigs, can be an invaluable tool for human medical research. Although the recent development of immunodeficient pigs has opened the door for the development of such a model, the efficient engraftment and differentiation of human cells may be difficult to achieve. The transplantation of human cells into fetal pigs, whose immune system is immature, will ameliorate this problem. Therefore, we examined the development of porcine fetal thymus, which is critical for the establishment of the immune system. We first analyzed the levels of mRNA expression of genes that are relevant to the function of thymic epithelial cells or thymocytes in whole thymi from 35 to 85 days of gestation (DG) and at 2 days postpartum (DP) by quantitative RT-PCR. In addition, immunohistochemical analyses of thymic epithelial cells from DG35 to DG55 and DP2 were performed. These analyses showed that the thymic cortex was formed as early as DG35, and thymic medulla gradually developed from DG45 to DG55. These findings suggested that, at least before DG45, the thymus do not differentiate to form fully functional T cells.

Keywords :
fetal pig, gene expression, immunohistochemistry, thymus

Date Deposited : 07 Apr 2015 10:10

Last Modified : 07 Apr 2015 10:10

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 63, Number 2, - 2014

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