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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 3 > Hitoki Yamanaka, Toshikazu Takagi, Makiko Ohsawa, Naoto Yamamoto, Noriaki Kubo, Takahira Takemoto, Shoko Sasano, Ritsuko Masuyama, Kazutaka Ohsawa

Identification and Characterization ofVancomycin-resistant Enterococcus species Frequently Isolated from Laboratory Mice

Hitoki Yamanaka, Toshikazu Takagi, Makiko Ohsawa, Naoto Yamamoto, Noriaki Kubo, Takahira Takemoto, Shoko Sasano, Ritsuko Masuyama, Kazutaka Ohsawa
1)Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan 2)Department of Molecular Bone Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan
Abstract :

To determine the prevalence of drug resistant bacteria colonizing laboratory mice, we isolated and characterized vancomycin-resistant Enterococcus species (VRE) from commercially available mice. A total of 24 VRE isolates were obtained from 19 of 21 mouse strains supplied by 4 commercial breeding companies. Of these, 19 isolates of E. gallinarum and 5 isolates of E. casseliflavus possessing the vanC1 and vanC2/3 genes intrinsically, exhibited intermediate resistance to vancomycin respectively. In addition, these isolates also exhibited diverse resistant patterns to erythromycin, tetracycline, and ciprofloxacin, whereas the use of antibiotics had not been undertaken in mouse strains tested in this study. Although 6 virulence-associated genes (ace, asa, cylA, efaA, esp, and gelE) and secretion of gelatinase and hemolysin were not detected in all isolates, 23 of 24 isolates including the isolates of E. casselifalvus secreted ATP into culture supernatants. Since secretion of ATP by bacteria resident in the intestinal tract modulates the local immune responses, the prevalence of ATP-secreting VRE in mice therefore needs to be considered in animal experiments that alter the gut microflora by use of antibiotics.

Keywords :
ATP, laboratory mice, prevalence, vancomycin-resistant Enterococcus , virulence factor

Date Deposited : 07 Apr 2015 10:23

Last Modified : 07 Apr 2015 10:23

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 63, Number 3, - 2014

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