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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 4 > Ying PENG, Chunfu WU, Jingyu YANG, Xiaobo LI Released: October 30, 2014

Gut Microbial Diversity in Rat Model Induced by Rhubarb

Ying PENG, Chunfu WU, Jingyu YANG, Xiaobo LI Released: October 30, 2014
1)School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, P.R. China 2)School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, P.R. China
Abstract :

Rhubarb is often used to establish chronic diarrhea and spleen (Pi)-deficiency syndrome animal models in China. In this study, we utilized the enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) method to detect changes in bacterial diversity in feces and the bowel mucosa associated with this model. Total microbial genomic DNA from the small bowel (duodenum, jejunum, and ileum), large bowel (proximal colon, distal colon, and rectum), cecum, and feces of normal and rhubarb-exposed rats were used as templates for the ERIC-PCR analysis. We found that the fecal microbial composition did not correspond to the bowel bacteria mix. More bacterial diversity was observed in the ileum of rhubarb-exposed rats (P<0.05). Furthermore, a 380 bp product was found to be increased in rhubarb-exposed rats both in faces and the bowel mucosa. The product was cloned and sequenced and showed high similarity with regions of the Bacteroides genome. AS a result of discriminant analysis with the SPSS software, the Canonical Discriminant Function Formulae for model rats was established.

Keywords :
animal model, bacterial diversity, ERIC-PCR, rhubarb

Date Deposited : 07 Apr 2015 14:35

Last Modified : 07 Apr 2015 14:35

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/62/1/_contents

Volume 63, Number 4, - 2014

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