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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2014 > Volume 63 Number 4 > Li WU, Chen WANG, Xiuzhong YAO, Kai LIU, Yanjun XU, Haitao ZHANG, Caixia FU, Xiaolin WANG, Yingyi LI

Application of 3.0 Tesla Magnetic Resonance Imaging for Diagnosis in the Orthotopic Nude Mouse Model of Pancreatic Cancer

Li WU, Chen WANG, Xiuzhong YAO, Kai LIU, Yanjun XU, Haitao ZHANG, Caixia FU, Xiaolin WANG, Yingyi LI
Department of Intervetional Radiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 20032, P.R.China
Abstract :

The aim of this study was to successfully establish an orthotopic murine model using two different human pancreatic adenocarcinoma cell lines and to propose a 3.0 tesla MRI protocol for noninvasive characterization of this model. SW1990 and MIAPaca-2 tumor cells were injected into the pancreas of BALB/C nu/nu mice. Tumor growth rate and morphological information were assessed by 3.0 tesla MRI (T1WI, T2WI and DCE-MRI) and immunohistology. Proliferation of SW1990 was significantly faster than that of MIAPaca-2 (P=0.000), but MIAPaca-2 mice had a significantly shorter survival than SW1990 mice (41 days and 44 days respectively, P=0.027). MRI could reliably monitor tumor growth in both cell lines: the tumors exhibiting a spherical growth pattern showed a high-intensity signal, and the SW1990 group developed significantly larger tumors compared with the MIAPaCa-2 group. There were no statistical differences between the two groups in which tumor size was assessed using electronic calipers and an MRI scan (P=0.680). Both tumors showed a slow gradual enhancement pattern. Immunohistochemistry demonstrated tumor tissues showing high expression of Ki-67. This model closely mimics human pancreatic cancer and permits monitoring of tumor growth and morphological information by noninvasive 3.0 tesla MRI studies reducing the number of mice required.

Keywords :
MIAPaCa-2, MRI, orthotopic murine model, pancreatic cancer, SW1990

Date Deposited : 07 Apr 2015 14:37

Last Modified : 07 Apr 2015 14:37

Official URL: http://

Volume 63, Number 4, - 2014

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