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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 1 > Cristina LEMINI, Ruth JAIMEZ, Alejandra FIGUEROA, Lucía MARTINEZ-MOTA, María Estela AVILA, Martha MEDINA

Ovariectomy differential influence on some hemostatic markers of mice and rats

Cristina LEMINI, Ruth JAIMEZ, Alejandra FIGUEROA, Lucía MARTINEZ-MOTA, María Estela AVILA, Martha MEDINA
1)Facultad de Medicina, Departamento de Farmacología, Universidad Nacional Autónoma de México, Av. Universidad 3000, Ciudad Universitaria, CP 04510, México D.F., México 2)Laboratorio de Farmacología Conductual, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, CP 14370, México D.F., México
Abstract :

Rodent ovariectomy is an experimental method to eliminate the main source of sexual steroids. This work explored for the first time the ovariectomy temporal changes induced in the hemostatic coagulation markers: prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen concentration (FIB) along with uterine weight on adult female CD1 mice and Wistar rats. Uterine weight (Uw) was assessed before ovariectomy (control), and 1, 3, 5, 7, 9, 16, and 21 days after surgery. PT, aPTT, TT and FIB were estimated the same days, using reported standard techniques. Ovariectomy decreased Uw, since day 1; and from day 10 to 21 reached the lowest values for both species. After day 1, mice hemostatic parameters changed (PT +10%, P<0.05; aPTT +53%, P<0.05; TT −24%, P<0.05; FIB +67%, P<0.05). Rats showed significant changes only in TT and FIB (TT −13%, P<0.001; FIB +65%, P<0.001). Neither mice PT, aPTT and TT, recovered control values after 21 days. In the rats from day 5 to 16 aPTT diminished (18–23%, P<0.05) recovering to control values on day 21, TT after 9 days and PT on day 16. In both species, FIB returned to its control values after 9 days. Ovariectomy differentially altered the PT hemostatic parameter of mice and rats indicating a non-equivalence among both species behaviour for experimental studies of blood coagulation.

Keywords :
blood coagulation, hemostatic parameters, mice, ovariectomy, rat

Date Deposited : 08 Apr 2015 09:46

Last Modified : 08 Apr 2015 09:46

Official URL: http://

Volume 64, Number 1, - 2015

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