UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2011 > Volume 7 Number 9 > Minerva Arce-Fonseca1,2,*, Angel Ramos-Ligonio1,3, *, Aracely López-Monteón1,3, Berenice Salgado-Jiménez1, Patricia Talamás-Rohana1, José Luis Rosales-Encina1,✉

A DNA Vaccine Encoding for TcSSP4 Induces Protection against Acute and Chronic Infection in Experimental Chagas Disease

Minerva Arce-Fonseca1,2,*, Angel Ramos-Ligonio1,3, *, Aracely López-Monteón1,3, Berenice Salgado-Jiménez1, Patricia Talamás-Rohana1, José Luis Rosales-Encina1,✉
1. Departamento de Infectómica y Patogenesis Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., México D.F. 07360, México 2. Departamento de Biología Molecular, Instituto Nacional de Cardiología “Ignacio Chávez”, México D.F.14080, México. 3. LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, 94340, México.
Abstract :

Immunization of mice with plasmids containing genes of Trypanosoma cruzi induces protective immunity in the murine model of Chagas disease. A cDNA clone that codes for an amastigote-specific surface protein (TcSSP4) was used as a candidate to develop a DNA vaccine. Mice were immunized with the recombinant protein rTcSSP4 and with cDNA for TcSSP4, and challenged with bloodstream trypomastigotes. Immunization with rTcSSP4 protein makes mice more susceptible to trypomastigote infection, with high mortality rates, whereas mice immunized with a eukaryotic expression plasmid containing the TcSSP4 cDNA were able to control the acute phase of infection. Heart tissue of gene-vaccinated animals did not show myocarditis and tissue damage at 365 days following infection, as compared with control animals. INF-γ was detected in sera of DNA vaccinated mice shortly after immunization, suggesting the development of a Th1 response. The TcSSP4 gene is a promising candidate for the development of an anti-T. cruzi DNA vaccine.

Keywords :
DNA immunization, Trypanosoma cruzi, TcSSP4

Date Deposited : 08 Apr 2015 12:58

Last Modified : 08 Apr 2015 12:58

Official URL: http://www.ijbs.com/ms/archive

Volume 7, Number 9, - 2011 , ISSN 1545-1003

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal