International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2012 > Volume 8 Number 2 > Florence Brellier1*, Enrico Martina1,2*, Matthias Chiquet3, Jacqueline Ferralli1, Michael van der Heyden4, Gertraud Orend4, Johannes C. Schittny5, Ruth Chiquet-Ehrismann1,2, Richard P. Tucker6,✉
The Adhesion Modulating Properties of Tenascin-W
Florence Brellier1*, Enrico Martina1,2*, Matthias Chiquet3, Jacqueline Ferralli1, Michael van der Heyden4, Gertraud Orend4, Johannes C. Schittny5, Ruth Chiquet-Ehrismann1,2, Richard P. Tucker6,✉
1. Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, CH-4058 Basel, Switzerland. 2. University of Basel, Faculty of Science, CH-4056 Basel, Switzerland. 3. Department of Orthodontics and Dentofacial Orthopedics, University of Bern, CH-3010 Bern, Switzerland. 4. Inserm, U682, Strasbourg, F-67200 France, University Strasbourg, UMR-S682, Strasbourg F-67081, France. 5. Institute of Anatomy, University of Bern, CH-3000 Bern, Switzerland. 6. Department of Cell Biology and Human Anatomy, University of California at Davis, Davis, California 95616 USA.
Tenascins are extracellular matrix glycoproteins associated with cell motility, proliferation and differentiation. Tenascin-C inhibits cell spreading by binding to fibronectin; tenascin-R and tenascin-X also have anti-adhesive properties in vitro. Here we have studied the adhesion modulating properties of the most recently characterized tenascin, tenascin-W. C2C12 cells, a murine myoblast cell line, will form broad lamellipodia with stress fibers and focal adhesion complexes after culture on fibronectin. In contrast, C2C12 cells cultured on tenascin-W fail to spread and form stress fibers or focal adhesion complexes, and instead acquire a multipolar shape with short, actin-tipped pseudopodia. The same stellate morphology is observed when C2C12 cells are cultured on a mixture of fibronectin and tenascin-W, or on fibronectin in the presence of soluble tenascin-W. Tenascin-W combined with fibronectin also inhibits the spreading of mouse embryo fibroblasts when compared with cells cultured on fibronectin alone. The similarity between the adhesion modulating effects of tenascin-W and tenascin-C in vitro led us to study the possibility of tenascin-W compensating for tenascin-C in tenascin-C knockout mice, especially during epidermal wound healing. Dermal fibroblasts harvested from a tenascin-C knockout mouse express tenascin-W, but dermal fibroblasts taken from a wild type mouse do not. However, there is no upregulation of tenascin-W in the dermis of tenascin-C knockout mice, or in the granulation tissue of skin wounds in tenascin-C knockout animals. Similarly, tenascin-X is not upregulated in early wound granulation tissue in the tenascin-C knockout mice. Thus, tenascin-W is able to inhibit cell spreading in vitro and it is upregulated in dermal fibroblasts taken from the tenascin-C knockout mouse, but neither it nor tenascin-X are likely to compensate for missing tenascin-C during wound healing.
tenascin, extracellular matrix, fibronectin, wound healing, C2C12.
Date Deposited : 09 Apr 2015 10:36
Official URL: http://www.ijbs.com/ms/archive
Last Modified : 09 Apr 2015 10:36
Volume 8, Number 2, - 2012 , ISSN 1545-1003
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