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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2012 > Volume 8 Number 3 > Anjali Jaiprakash1*, Indira Prasadam1*, Jian Q. Feng3, Ying Liu3, Ross Crawford1, 2, Yin Xiao1✉

Phenotypic Characterization of Osteoarthritic Osteocytes from the Sclerotic Zones: A Possible Pathological Role in Subchondral Bone Sclerosis

Anjali Jaiprakash1*, Indira Prasadam1*, Jian Q. Feng3, Ying Liu3, Ross Crawford1, 2, Yin Xiao1✉
1. Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia 2. Prince Charles Hospital, Brisbane, Queensland, Brisbane, Australia 3. Texas A&M Health Science Center, Baylor College of Dentistry, Texas, USA
Abstract :

Subchondral bone sclerosis is a well-recognised manifestation of osteoarthritis (OA). The osteocyte cell network is now considered to be central to the regulation of bone homeostasis; however, it is not known whether the integrity of the osteocyte cell network is altered in OA patients. The aim of this study was to investigate OA osteocyte phenotypic changes and its potential role in OA subchondral bone pathogenesis. The morphological and phenotypic changes of osteocytes in OA samples were investigated by micro-CT, SEM, histology, immunohistochemistry, TRAP staining, apoptosis assay and real-time PCR studies. We demonstrated that in OA subchondral bone, the osteocyte morphology was altered showing rough and rounded cell body with fewer and disorganized dendrites compared with the osteocytes in control samples. OA osteocyte also showed dysregulated expression of osteocyte markers, apoptosis, and degradative enzymes, indicating that the phenotypical changes in OA osteocytes were accompanied with OA subchondral bone remodelling (increased osteoblast and osteoclast activity) and increased bone volume with altered mineral content. Significant alteration of osteocytes identified in OA samples indicates a potential regulatory role of osteocytes in subchondral bone remodelling and mineral metabolism during OA pathogenesis.

Keywords :
osteocyte, osteoarthritis, subchondral bone, sclerosis, DMP1 and SOST

Date Deposited : 09 Apr 2015 10:55

Last Modified : 09 Apr 2015 10:55

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Volume 8, Number 3, - 2012 , ISSN 1545-1003

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