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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2012 > Volume 8 Number 4 > Changsong Zhang1,2,4*, Yang Ling1*, Chenghui Zhang3*, Yun Xu3, Lu Gao2, Rong Li2, Jing Zhu1, Lieying Fan4, Lixin Wei2 ✉

The Silencing of RECK Gene is Associated with Promoter Hypermethylation and Poor Survival in Hepatocellular Carcinoma

Changsong Zhang1,2,4*, Yang Ling1*, Chenghui Zhang3*, Yun Xu3, Lu Gao2, Rong Li2, Jing Zhu1, Lieying Fan4, Lixin Wei2 ✉
1. Clinical Oncology Laboratory, Changzhou Tumor Hospital, Soochow University, Changzhou, China; 2. Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai, China; 3. Department of Oncology, Nanyang Center Hospital, Nanyang, China; 4. Department of Clinical Laboratory, Shanghai East Hospital, Tongji University Medical School, Shanghai, China.
Abstract :

Background: To evaluate the promoter methylation status of RECK gene and mRNA expression in patients with hepatocellular carcinoma (HCC).

Methods: We analyzed RECK methylation by MSP, and RECK mRNA by real-time PCR in 74 HCC. The liver cell lines (7721, Chang and Hep-G2) were treated with 5-Aza-CdR and TSA.

Results: RECK mRNA were lower in HCC tissues (Mean -∆Ct = -3.29) than that in Non-Hcc tissues (Mean -∆Ct = -2.42). Expression of RECK was elevated in only 24 (32.43%) of the 74 HCC patients but decreased (-∆∆Ct<0) in 50 (67.57%) of the patients. RECK promoter was hypermethylated in 55.4% (41/74) of HCCs, and in only 17.6% (13/74) of Non-Hcc samples. RECK mRNA were lower in HCC patients with hypermethylation (∆MI>=0.5) (Mean -∆∆Ct = -1.75) than those with demethylation (∆MI<0.5) (Mean -∆∆Ct = 0.05), and there is a decreased tendency for RECK mRNA in HCC patients with promoter hypermethylation (p = 0.002). There was a significantly correlation found between RECK mRNA and poor survival after surgery. After treated by 5-Aza-CdR and TSA, we found that RECK mRNA induced different changes in 7721, Chang and Hep-G2 cells. And RECK demethylation also induced by epigenetic inhibitors.

Conclusion: The results suggested that the hypermethylation may lead to promoter silencing of RECK mRNA and associated with poor survival in HCC.

Keywords :
RECK, hepatocellular carcinoma, methylation.

Date Deposited : 09 Apr 2015 11:02

Last Modified : 09 Apr 2015 11:02

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Volume 8, Number 4, - 2012 , ISSN 1545-1003

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