International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2012 > Volume 8 Number 10 > Yunxue Guo1, Yaosheng Chen1,2, Yun Zhang1, Yue Zhang1, Luxi Chen1, Delin Mo1,2✉
Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1
Yunxue Guo1, Yaosheng Chen1,2, Yun Zhang1, Yue Zhang1, Luxi Chen1, Delin Mo1,2✉
1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China; 2. Guangdong Province Engineering and technology R&D centers for pig breeding, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China.
enerally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.
miR-145, IRS1, inhibit, dedifferentiated fat cells, adipogenesis.
Date Deposited : 09 Apr 2015 14:54
Official URL: http://www.ijbs.com/ms/archive
Last Modified : 09 Apr 2015 14:54
Volume 8, Number 10, - 2012 , ISSN 1545-1003
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