International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2013 > Volume 9 Number 4 > Yehui Xiong, Hongmei Zeng✉, Yuliang Zhang, Dawei Xu, Dewen Qiu✉
Silencing the HaHR3 Gene by Transgenic Plant-mediated RNAi to Disrupt Helicoverpa armigera Development
Yehui Xiong, Hongmei Zeng✉, Yuliang Zhang, Dawei Xu, Dewen Qiu✉
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
RNA interference (RNAi) caused by exogenous double-stranded RNA (dsRNA) has developed into a powerful technique in functional genomics, and to date it is widely used to down-regulate crucial physiology-related genes to control pest insects. A molt-regulating transcription factor gene, HaHR3, of cotton bollworm (Helicoverpa armigera) was selected as the target gene. Four different fragments covering the coding sequence (CDS) of HaHR3 were cloned into vector L4440 to express dsRNAs in Escherichia coli. The most effective silencing fragment was then cloned into a plant over-expression vector to express a hairpin RNA (hpRNA) in transgenic tobacco (Nicotiana tabacum). When H. armigera larvae were fed the E. coli or transgenic plants, the HaHR3 mRNA and protein levels dramatically decreased, resulting developmental deformity and larval lethality. The results demonstrate that both recombinant bacteria and transgenic plants could induce HaHR3 silence to disrupt H. armigera development, transgenic plant-mediated RNAi is emerging as a powerful approach for controlling insect pests.
RNAi, Helicoverpa armigera, HaHR3, transgenic tobacco plants, systemic RNAi.
Date Deposited : 09 Apr 2015 15:50
Official URL: http://www.ijbs.com/ms/archive
Last Modified : 09 Apr 2015 15:50
Volume 9, Number 4, - 2013 , ISSN 1545-1003
Full Text Original