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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2013 > Volume 9 Number 7 > Bing-rong ZHOU*, Xian-fei GUO*, Jia-an ZHANG, Yang XU, Wei LI, Di WU, Zhi-qiang YIN, Felicia Permatasari, Dan LUO✉

Elevated miR-34c-5p Mediates Dermal Fibroblast Senescence by Ultraviolet Irradiation

Bing-rong ZHOU*, Xian-fei GUO*, Jia-an ZHANG, Yang XU, Wei LI, Di WU, Zhi-qiang YIN, Felicia Permatasari, Dan LUO✉
Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Guangzhou road 300#, Nanjing, Jiangsu province, China PR.
Abstract :

Previous studies showed that several miRNAs can regulate pathways involved in UVB-induced premature senescence and response to ultraviolet irradiation. It has also been reported that miR-34c-5p may be involved in senescence-related mechanisms. We propose that miR-34c-5p may play a crucial role in senescence of normal human primary dermal fibroblasts. Here, we explored the roles of miR-34c-5p in UVB-induced premature senescence on dermal fibroblasts. MiR-34c-5p expression was increased in dermal fibroblasts after repeated subcytotoxic UVB treatments. Underexpression of miR-34c-5p in dermal fibroblasts led to a marked delay of many senescent phenotypes induced by repeated UVB treatments. Furthermore, underexpression of miR-34c-5p in dermal fibroblasts can antagonize the alteration of G1-arrested fibroblasts. Moreover, E2F3, which can inactivate p53 pathway and play a role in cell cycle progression, is a down-stream target of miR-34c-5p. Forced down-expression of miR-34c-5p decreased the expression of UVB-SIPS induced P21 and P53 at both mRNA and protein levels. Our data demonstrated that down-regulation of miR-34c-5p can protect human primary dermal fibroblasts from UVB-induced premature senescence via regulations of some senescence-related molecules.

Keywords :
miR-34c-5p, UVB, premature senescence, human skin fibroblasts.

Date Deposited : 11 Apr 2015 10:30

Last Modified : 11 Apr 2015 10:30

Official URL: http://www.ijbs.com/ms/archive

Volume 9, Number 7, - 2013 , ISSN 1449-2288

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