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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2013 > Volume 9 Number 10 > Massimo Marrelli1,2*, Francesco Paduano2*, Marco Tatullo1*,2✉

Cells Isolated from Human Periapical Cysts Express Mesenchymal Stem Cell-like Properties

Massimo Marrelli1,2*, Francesco Paduano2*, Marco Tatullo1*,2✉
1. Unit of Maxillofacial Surgery, Calabrodental, Crotone, Italy; 2. Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
Abstract :

We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73+, CD90+, CD105+, CD13+, CD29+, CD44+, CD45-, STRO-1+, CD146+) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast- and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.

Keywords :
Mesenchymal stem cells (MSCs), cyst, periapical inflammatory tissues, mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs), osteogenic differentiation, adipogenic differentiation, odontogenic cyst.

Date Deposited : 11 Apr 2015 11:28

Last Modified : 11 Apr 2015 11:28

Official URL: http://www.ijbs.com/ms/archive

Volume 9, Number 10, - 2013 , ISSN 1449-2288

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