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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2011 > Volume 60 Number 1 > Hideharu OCHIAI1), Ken ONDA2), Takuya MARUO3) and Nobuyuki KANEMAKI3)

Aquaporin-1 Expression in Canine Peripheral Erythrocytes and Its Relation to Cell Volume

Hideharu OCHIAI1), Ken ONDA2), Takuya MARUO3) and Nobuyuki KANEMAKI3)
1) Research Institute of Biosciences, Azabu University 2) Laboratory of Veterinary Internal Medicine III, Azabu University 3) Animal Teaching Hospital, Azabu University
Abstract :

To evaluate the relationship between aquaporin-1 (AQP1) expression and the cell volume of red blood cells (RBCs), canine peripheral RBCs were separated according to specific gravity, and expression of the AQP1 protein on the membrane of RBCs was compared using anti-dog AQP1 polypeptide serum. Western blot analysis indicated that there was no significant difference in AQP1 expression between large and small cell fractions. In addition, the AQP1 expression of inherited high K/low Na RBCs which are known to be 20% larger than normal RBCs, was comparable to that of normal RBCs. These results suggest that AQP1, the major water channel in RBCs, does not determine the cell volume of peripheral canine RBCs.

Keywords :
aquaporin-1, cell volume, RBCs

Date Deposited : 21 Jun 2011 11:26

Last Modified : 21 Jun 2011 11:26

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Volume 60, Number 1, - 2011

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