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The Journal of Poultry Science

Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds

Shusei Mizushima
Presentaddress:DepartmentofAppliedBiologicalChemistry,FacultyofAgriculture,ShizuokaUniversity
Abstract :

In mammals, a normal offspring can be obtained even from infertile male by intracytoplasmic sperm injection (ICSI). Although ICSI technique has permitted significant progress in clinical practice in humans and mouse to date, it has been established recently in birds. In addition, efficiency of fertility and developmental rates has been low and no chick has been produced by in vitro fertilization and culture. Furthermore, polyspermic fertilization and subsequent normal developmental processes remains unknown. The enhancement of fertility and developmental rates is the first step in the avian ICSI system to be applied for protection of endangered species and production of transgenic and clone birds. This review paper describes (1) the establishment of ICSI technique in Japanese quail, (2) molecular mechanisms whereby polyspermy activates development of quail oocyte, (3) improvement of ICSI efficiency by phospholipase Cζ cRNA. Also, possible application of ICSI for avian sex manipulation and transgenic birds was summarized.

Keywords :
viansexmanipulation,ICSI,Japanesequail,phospholipaseC ζ ,polyspermy,transgenicbirds

Date Deposited : 07 May 2015 11:11

Last Modified : 07 May 2015 11:11

Official URL: http://https://www.jstage.jst.go.jp/browse/jpsa/48/3/_contents

Volume 49, Number 4, - 2012 , ISSN 1346-7395

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