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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2011 > Volume 60 Number 2 > Takehisa HEBISHIMA1)3)6), Yasunobu MATSUMOTO3), Gen WATANABE1)6), Gen-ichiro SOMA4)5), Chie KOHCHI4)5), Kazuyoshi TAYA1)6), Yoshihiro HAYASHI3) and Yoshikazu HIROTA2)6)

Oral Administration of Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) Improves the Survival of B16 Melanoma-Inoculated Model Mice

Takehisa HEBISHIMA1)3)6), Yasunobu MATSUMOTO3), Gen WATANABE1)6), Gen-ichiro SOMA4)5), Chie KOHCHI4)5), Kazuyoshi TAYA1)6), Yoshihiro HAYASHI3) and Yoshikazu HIROTA2)6)
1) Laboratories of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology 2) Laboratories of Veterinary Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology 3) Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, University of Tokyo 4) Institute for Health Sciences, Tokushima Bunri University 5) Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University 6) The United Graduated School of Veterinary Sciences, Gifu University
Abstract :

To investigate the usefulness of the immunopotentiator from Pantoea agglomerans 1 (IP-PA1) as a supportive drug in melanoma therapy, we analyzed the immunological effects of IP-PA1 on melanoma-inoculated model mice. Oral administration of IP-PA1 increased the serum levels of tumor necrosis factor (TNF)-α at 2 h after the administration and interferon (IFN)-γ and IL-12 at 12 h after the administration in naïve BALB/cCrSlc mice as evaluated by ELISA. IP-PA1 did not affect the proliferation of melanoma cells directly determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Combinatory treatment of IP-PA1 with doxorubicin for 9 days increased the serum levels of IFN-γ and IL-12 by 71.0 and 15.3%, respectively, compared to the treatment of doxorubicin alone in melanoma-bearing C57BL/6NCrSlc mice as evaluated by ELISA. It also increased the proportion of natural killer (NK) cells and the ratio of CD4+ to CD8+ T cells in the spleen from 6.1 ± 0.3 to 7.4 ± 0.5% and from 1.25 ± 0.03 to 1.38 ± 0.04, respectively, compared to the treatment of doxorubicin alone as analyzed by flow cytometry. The mean survival period of melanoma-bearing, doxorubicin treated mice was prolonged from 31.4 ± 7.1 to 35.3 ± 8.4, 51.1 ± 5.4, and 45.0 ± 8.4 days by combinatory treatment of IP-PA1 at the daily doses of 0.1, 0.5, and 1 mg/kg, respectively. In conclusion, the results of the present study suggest the usefulness of IP-PA1 as a supportive drug in melanoma therapy.

Keywords :
chemotherapy, immunomodulator, immunosuppression, IP-PA1, melanoma

Date Deposited : 21 Jun 2011 11:32

Last Modified : 21 Jun 2011 11:32

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 60, Number 2, - 2011

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