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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 2 > Takashi KURAMOTO1), Mayuko YOKOE1), Kayoko YAGASAKI1), Tatsuya KAWAGUCHI1), Kenta KUMAFUJI1) and Tadao SERIKAWA1)

Genetic Analyses of Fancy Rat-Derived Mutations

Takashi KURAMOTO1), Mayuko YOKOE1), Kayoko YAGASAKI1), Tatsuya KAWAGUCHI1), Kenta KUMAFUJI1) and Tadao SERIKAWA1)
1) Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University
Abstract :

To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (am), Black eye (Be), grey (g), Pearl (Pel), siamese (sia); 1 coat pattern, head spot (hs); 2 coat textures, Rex (Re), satin (sat); and an ear pinnae malformation, dumbo (dmbo). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): am to Chr 1, sia to Chr 1, sat to Chr 3, Re to Chr 7, g to Chr 8, dmbo to Chr 14, and hs to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for sia. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.

Keywords :
bioresource, coat color, genetic mapping, inbreeding, mutation

Date Deposited : 21 Jun 2011 13:42

Last Modified : 21 Jun 2011 14:19

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 2, - 2010

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