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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2015 > Volume 14 Number 4 > Hazem Ramadan , Byungjin Min , Amit K. Tiwari , Gopal Reddy , 1,2,4 1 1,5 1 Abiodun Adesiyun , Arthur Hinton Jr. and Woubit Abdela 3 4 1

Antibacterial Activity of Pomegranate, Orange and Lemon Peel Extracts Against Food-Borne Pathogens and Spoilage Bacteria In vitro and on Poultry Skin

Hazem Ramadan , Byungjin Min , Amit K. Tiwari , Gopal Reddy , 1,2,4 1 1,5 1 Abiodun Adesiyun , Arthur Hinton Jr. and Woubit Abdela 3 4 1
College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, AL 36088, USA 1 Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, 2 Mansoura University, Mansoura 35516, Egypt Department of Paraclinical Sciences, School of Veterinary Medicine, Faculty of Medical Sciences, 3 University of the West Indies, St. Augustine, Trinidad and Tobago Poultry Microbiological Safety and Processing Research Unit, Russell Research Center, 4 U.S. National Poultry Research Center, Athens, GA 30605, USA Department of Pharmacology and Experimental Therapeutics, 5 College of Pharmacy and Pharmaceutical Sciences, University of Toledo, OH 43614, USA
Abstract :

This study was conducted to determine the antimicrobial activity of methanol and ethanol extracts of peels of pomegranate (Punica grana), orange (Citrus siensis) and lemon (Limona taris) against four foodborne pathogens (Listeria monocytogenes, Salmonella Typhimurium, Escherichia coli and methicillinresistant Staphylococcus aureus (MRSA) and a food spoilage bacterium (Pseudomonas fluorescens. Inhibition tests were conducted in vitro using the disc diffusion and minimum inhibition concentration (MIC) assays with the Bioscreen Microbiology analyzer. The study also evaluated the antimicrobial activity of the extracts in situ by determining CFU/ml of bacteria recovered from rinsates of chicken skin treated with the peel extracts and by examining the microflora of treated skin samples using scanning electron microscopy (SEM). The antimicrobial activity of all extracts, except the pomegranate ethanol extract, were dependent on the concentration of extract that the bacteria were exposed to during the trials. Treating the inoculated chicken skin with 5 mg/ml of either the five extracts produced significant (p<0.01) reductions in CFU/ml of MRSA, L. monocytogenes and P. fluorescens recovered and the MRSA findings were supported by SEM observations. The antimicrobial activity of peel extracts of pomegranate, orange and lemon indicates that these extracts may be used as sanitizers to reduce microbial contamination of some foods and processing.

Keywords :
Plant extracts, antimicrobial activity, foodborne pathogens, spoilage bacterium, chicken skin

Date Deposited : 31 Dec 2015 11:52

Last Modified : 31 Dec 2015 11:52

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Volume 14, Number 4, - 2015 , ISSN 1682-8356

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