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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2015 > Volume 14 Number 5 > Hazem Ramadan , Charlene Jackson and Arthur Hinton, Jr. 1 2 3

Screening and Rapid Identification of Campylobacter Spp. DNA by FlaA PCR Based Method on Chicken and Human Fecal Samples in Egypt

Hazem Ramadan , Charlene Jackson and Arthur Hinton, Jr. 1 2 3
Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, 1 Mansoura University, Mansoura 35516, Egypt Bacterial Epidemiology and Antimicrobial Resistance Research Unit, 2 Russell Research Center, Athens, GA 30605, USA Poultry Microbiological Safety and Processing Research Unit, Russell Research Center, 3 U.S. National Poultry Research Center, Athens, GA 30605, USA
Abstract :

Due to culturability requirements encountered by the conventional isolation of Campylobacter spp., rapid molecular techniques for its direct identification from clinical samples are useful. In this study, Campylobacter spp. DNA from human stool and chicken fecal samples were detected by flagellin gene (flaA) PCR. A total of 297 samples consisting of 163 adult human stools (102 from diarrheic patients and 61 from healthy persons) and 134 chicken feces were subjected to flaA PCR. Ten reference strains of Campylobacter spp. were included in this study as positive controls. Thirteen stool samples (7.98%) from the human fecal samples and 39 chicken fecal samples (29.1%) yielded the genus specific 1.7 Kb amplicon of Campylobacter spp. Eight (7.84%) diarrheic human stool specimens out of 102 samples and 5 (8.2%) apparently healthy human stool specimens out of 61 samples were positive by flaA PCR assay. All the Campyloacter reference strains examined giving the specific amplicon of 1.7 Kb. The existence of Campylobacter spp. DNA detected by flaA PCR in poultry and human samples taken from locations of Egypt highlights the zoonotic potential of Campylobacter. To the best of our knowledge, this is the first report in Egypt that uses flaA PCR as a rapid screening method for the direct detection of Campylobacter spp. from human and chicken feces

Keywords :
Campylobacter, human stool, poultry feces, flaA PCR

Date Deposited : 31 Dec 2015 11:59

Last Modified : 31 Dec 2015 11:59

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Volume 14, Number 5, - 2015 , ISSN 1682-8356

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