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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 2 > Haru OKAMOTO1)2), Shinsuke UMEDA1), Takehiro NOZAWA3), Michihiro T. SUZUKI4), Yasuhiro YOSHIKAWA5), Etsuko T. MATSUURA6) and Takeshi IWATA1)

Comparative Proteomic Analyses of Macular and Peripheral Retina of Cynomolgus Monkeys (Macaca fascicularis)

Haru OKAMOTO1)2), Shinsuke UMEDA1), Takehiro NOZAWA3), Michihiro T. SUZUKI4), Yasuhiro YOSHIKAWA5), Etsuko T. MATSUURA6) and Takeshi IWATA1)
1) Division of Molecular & Cellular Biology, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center 2) Department of Advanced Biosciences, Ochanomizu University 3) Analytical Instrument Division, AMR Inc. 4) The Corporation for Production and Research of Laboratory Primates 5) Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo 6) The Natural/Applied Sciences Division, Ochanomizu University
Abstract :

The central region of the primate retina is called the macula. The fovea is located at the center of the macula, where the photoreceptors are concentrated to create a neural network adapted for high visual acuity. Damage to the fovea, e.g., by macular dystrophies and age-related macular degeneration, can reduce central visual acuity. The molecular mechanisms leading to these diseases are most likely dependent on the proteins in the macula which differ from those in the peripheral retina in expression level. To investigate whether the distribution of proteins in the macula is different from the peripheral retina, proteomic analyses of tissues from these two regions of cynomolgus monkeys were compared. Two-dimensional gel electrophoresis and mass spectrometry identified 26 proteins that were present only in the macular gel spots. The expression levels of five proteins, cone photoreceptor specific arrestin-C, γ-synuclein, epidermal fatty acid binding protein, tropomyosin 1α chain, and heterogeneous nuclear ribonucleoproteins A2/B1, were significantly higher in the macula than in the peripheral retina. Immunostaining of macula sections by antibodies to each identified protein revealed unique localization in the retina, retinal pigment epithelial cells and the choroidal layer. Some of these proteins were located in cells with higher densities in the macula. We suggest that it will be important to study these proteins to determine their contribution to the pathogenesis and progression of macula diseases.

Keywords :
Comparative Proteomic Analyses of Macular and Peripheral Retina of Cynomolgus Monkeys (Macaca fascicularis)

Date Deposited : 21 Jun 2011 13:45

Last Modified : 21 Jun 2011 14:12

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 2, - 2010

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