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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2016 > Volume 14 Number 7 > Giang Nguyen , Magda Ismail Abo-Samaha , Gopal Reddy , Mohammed Abdulrahman , 1 2, 3 2 2 Temesgen Samuel , Abiodun Adesiyun and Woubit Abdela 2 4 2

Improved Detection of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium from Raw Meat Products Using Conventional and Newly Developed TaqMan Assays

Giang Nguyen , Magda Ismail Abo-Samaha , Gopal Reddy , Mohammed Abdulrahman , 1 2, 3 2 2 Temesgen Samuel , Abiodun Adesiyun and Woubit Abdela 2 4 2
National Institute for Food Control, Ha Noi, Viet Nam, 1 Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, 2 Tuskegee University, Tuskegee, Alabama-36088, USA Department of Animal Husbandry and Animal Wealth Development, 3 Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt Department of Production Animal Studies, Faculty of Veterinary Science, 4 University of Pretoria, Onderstepoort, Pretoria, South Africa
Abstract :

The most frequent bacteria contaminating raw meat samples from local retail outlets were determined. Comparison was made between conventional culture method (BIOLOG) and newly developed PCR assays performed on bacterial isolates (PCR-C), directly on enriched broths (Direct PCR-E) or on DNA extracted from enriched broths (PCR-E) of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium. Among 150 meat samples (chicken, pork, turkey and beef) tested, Campylobacter, Proteus, Listeria and Salmonella were detected in 27.0, 25.0, 19.0 and 3.3%, respectively, by using BIOLOG. Among 150 samples analyzed, the frequency of detection for C. jejuni, L. monocytogenes and S. Typhimurium were 8.0, 2.7 and 2.0% by both the BIOLOG and PCR-C assays. PCR-C and BIOLOG detected the same number of positive samples, 12 for C. jejuni, 3 for S. Typhimurium, however from the total 4 L. monocytogenes, only 3 were isolated correctly by BIOLOG. Both Direct PCR-E and PCR-E detected 13, 2 and 1 positive samples for C. jejuni, S. Typhimurium and L. monocytogenes, respectively. TaqMan PCR assay on enriched broths produced the most rapid and inhibition-free results compared with Direct PCR-E and PCR-C. The PCRbased methods provided results more rapidly (1-2 days versus 6-7 days) and therefore are recommended for improved detection of these pathogens

Keywords :
Salmonella, Campylobacter, Listeria, meat, isolation, TaqMan assay

Date Deposited : 02 Jan 2016 10:46

Last Modified : 02 Jan 2016 10:46

Official URL: http://www.pjbs.org/ijps/14(7).htm

Volume 14, Number 7, - 2016 , ISSN 1682-8356

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