UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2016 > Volume 14 Number 9 > Brett Weathers , Scott L. Branton , Roymon Jacob , 1,2 3 4 Robert L. Taylor Jr , E. David Peebles and G. Todd Pharr 5 4 1

Expression of the Ephrin Receptor B2 in the Embryonic Chicken Bursa of Fabricius

Brett Weathers , Scott L. Branton , Roymon Jacob , 1,2 3 4 Robert L. Taylor Jr , E. David Peebles and G. Todd Pharr 5 4 1
Department of Basic Sciences, College of Veterinary Medicine, 1 Mississippi State University, Mississippi State, MS 39762, USA Animal Hospital of North Myrtle Beach, North Myrtle Beach, SC 29582, USA 2 USDA, Agricultural Research Service, Poultry Research Unit, Mississippi State, MS 39762, USA 3 Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA 4 Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA
Abstract :

Chicken B-cells develop in the bursa of Fabricius. To understand the bursal microenvironment guiding B-cell development, previous studies identified ephrin (Eph) receptor B2 (EphB2) gene transcripts in the embryonic bursa. We hypothesize that the EphB2 receptors and their ligands could initiate contacts between developing B-cells and epithelial cells in the embryonic bursal follicle. To address this hypothesis, additional basic studies were conducted to determine if EphB2 is expressed in the embryonic bursa. Therefore, EphB2 gene expression was examined with reverse transcriptase-PCR and western blotting in the bursa on embryonic days (ED) 15 and 18. The RT-PCR experiments with bursal cDNA amplified transcripts from the EphB2 gene, which was confirmed by nucleotide sequencing. The anti-EphB2 antibody recognized a protein of the expected 120 kDa molecular mass and gave an acceptable signal to noise ratio on whole tissue protein western blots from the ED15 and ED18 bursa. This information can be used to design experiments for determining the cell type (s) expressing the EphB2 protein in the embryonic bursa

Keywords :
Embryo, bursa of Fabricius, ephrin receptor B2

Date Deposited : 05 Jan 2016 08:57

Last Modified : 05 Jan 2016 08:57

Official URL: http://www.pjbs.org/ijps/14(9).htm

Volume 14, Number 9, - 2016 , ISSN 1682-8356

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal