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.: Home > Animal Science Papers and Reports > 2015 > Volume 33 Number 2 > Jernej Ogorevc1 , Sonja Prpar Mihevc1 , Jakob Hedegaard2 , Dušan Benčina1 , Peter Dovč

Transcriptomic response of goat mammary epithelial cells to Mycoplasma agalactiae challenge – a preliminary study

Jernej Ogorevc1 , Sonja Prpar Mihevc1 , Jakob Hedegaard2 , Dušan Benčina1 , Peter Dovč
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia 2 Department of Molecular Biology and Genetics, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark (Present address: Department of Molecular Medicine, Aarhus University Hospital, Skejby, Palle Jull-Jensens Boulevard 99, DK-8200 Aarhus N, Denmark)
Abstract :

Mycoplasma agalactiae (Ma) is one of the main aetiological agents of intramammary infections in small ruminants, causing contagious agalactia. To better understand the underlying disease patterns a primary goat mammary epithelial cell (pgMEC) culture was established from the mammary tissue and challenged with Ma. High-throughput mRNA sequencing was performed to reveal differentially expressed genes (DEG) at different time-points (3 h, 12 h, and 24 h) post infection (PI). The pathway enrichment analysis of the DEG showed that infection significantly affected pathways associated with immune response, steroid metabolism, fatty acid metabolism, apoptosis signalling, transcription regulation, and cell cycle regulation. Based on the results we suggest that mammary epithelial cells in vivo contribute to the immune system by the induced expression of cytokines and other chemotactic agents, activation of the complement system and apoptosis pathways, and expression of genes coding for antimicrobial molecules and peptides. In our study we attempted to interpret the detected transcriptomic changes in a biological context and infer mammary infection *This work was supported by the Slovenian Research Agency (ARRS) (Research programme P4-0220), and EADGENE, FP7 EU Network of excellence. J.O. and S.P. were supported through the young researchers’ scheme of ARRS. **Corresponding author: 156 resistance candidate genes, interesting for further validation. Additionally, the results represent comprehensive goat mammary transcriptome information and demonstrate the applicability of the comparative genomics approach for annotation of goat data, using transcriptome information of a closely related species (Bos taurus) as a reference.

Keywords :
cell culture / high-throughput RNA sequencing / mammary gland / mastitis / Mycoplasma agalactiae

Date Deposited : 06 Jan 2016 09:26

Last Modified : 06 Jan 2016 09:26

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Volume 33, Number 2, March 2015 , ISSN 109-117

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