UMM Logo

Universitas Muhammadiyah Malang

Free Download Journals Directory

Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 2 > Kazuki NAKAO1)2) and Kenichi INOUE1)

Cryopreservation for Broader Production of Transgenic Mice by DNA Injection into Zygotes

Kazuki NAKAO1)2) and Kenichi INOUE1)
1) Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology 2) Division of Molecular Genetics, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine
Abstract :

Mutant mice are indispensable to biological and medical research, and transgenic mouse production by DNA injection into zygotes has been an important method for producing these mice which are used to examine the over-expression of genes and to analyze gene transcriptional regulatory sequences. Recently, cryopreservation of zygotes by a simple vitrification method has become popular, saving the labor of isolating zygotes following each injection. However, the DNA injection technique requires training in the use of special equipment, and the injection cannot be accomplished routinely in every laboratory. The exchange of live mice also risks the propagation of common murine pathogens and possible escape of the animals; therefore the transfer of mice in frozen zygotes or embryos is recommended. Here we propose injecting DNA into frozen and thawed zygotes, refreezing them before transportation to any destination, where they can be thawed and developed into pups. The rate of transgenic mouse production using this method does not decrease significantly, even if zygotes are frozen and thawed before and after their DNA injection, and will make transgenic studies more popular on a worldwide scale.

Keywords :
cryopreservation, DNA injection, mouse, transgenic, vitrification

Date Deposited : 21 Jun 2011 13:57

Last Modified : 21 Jun 2011 14:15

Official URL:

Volume 59, Number 2, - 2010

Full Text Original
Abstract : pdf doc