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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 2 > Masami Tanaka1), Minoru Watanabe2), Izuru Yokomi3), Naoki Matsumoto3), Katsuko Sudo4), Hitoshi Satoh5), Tsuneo Igarashi6), Azusa Seki7), Hitoshi Amano8), Kiyoshi Ohura8), Kakei Ryu9), Shunichi Shibata10), Motohiko Nagayama11), and Jun-ichi Tanuma11)

Establishment of a novel dwarf rat strain: cartilage calcification insufficient (CCI) rats

Masami Tanaka1), Minoru Watanabe2), Izuru Yokomi3), Naoki Matsumoto3), Katsuko Sudo4), Hitoshi Satoh5), Tsuneo Igarashi6), Azusa Seki7), Hitoshi Amano8), Kiyoshi Ohura8), Kakei Ryu9), Shunichi Shibata10), Motohiko Nagayama11), and Jun-ichi Tanuma11)
1)Department of Food and Nutrition, Junior College Division, The University of Aizu, Aizu-Wakamatsu, Fukushima 965-8570, Japan 2)Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine, Kawasaki, Kanagawa 216-8511, Japan 3)Department of Pharmacology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan 4)Animal Research Center, Tokyo Medical University, Tokyo 160-8402, Japan 5)Laboratory of Tumor Cell Biology, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 108- 8639, Japan 6)Animal Care Co., Ltd., Tokyo 164-0001, Japan 7)Hamri Co., Ltd. Tokyo110-0005, Japan 8)Department of Pharmacology, Osaka Dental University, Osaka 573-1121, Japan 9)Department of Pharmacology, Showa University School of Medicine, Tokyo 142-8555, Japan 10) Maxillofacial Anatomy, Department of Maxillofacial Biology, Maxillofacial/Neck Reconstruction, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8549, Japan 11) Department of Oral Pathology, Asahi University School of Dentistry, Hozumi, Gifu 501-0296, Japan
Abstract :

Rats with dwarfism accompanied by skeletal abnormalities, such as shortness of the limbs, tail, and body (dwarf rats), emerged in a Jcl-derived Sprague-Dawley rat colony maintained at the Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine. Since the dwarfism was assumed to be due to a genetic mutation based on its frequency, we bred the dwarf rats and investigated their characteristics in order to identify the causative factors of their phenotypes and whether they could be used as a human disease model. One male and female that produced dwarf progeny were selected, and reproduction was initiated by mating the pair. The incidence of dwarfism was 25.8% among the resultant litter, and dwarfism occurred in both genders, suggesting that it was inherited in an autosomal recessive manner. At 12 weeks of age, the body weights of the male and female dwarf rats were 40% and 57% of those of the normal rats, respectively. In soft X-ray radiographic and histological examinations, shortening and hypoplasia of the long bones, such as the tibia and femur, were observed, which were suggestive of endochondral ossification abnormalities. An immunohistochemical examination detected an aggrecan synthesis disorder, which might have led to delayed calcification and increased growth plate thickening in the dwarf rats. We hypothesized that the principal characteristics of the dwarf rats were systemically induced by insufficient cartilage calcification in their long bones; thus, we named them cartilage calcification insufficient (CCI) rats.

Keywords :
animal model, cartilage, calcification, dwarf, rat

Date Deposited : 06 Jan 2016 11:24

Last Modified : 06 Jan 2016 11:24

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/2/_contents

Volume 64, Number 2, - 2015 , ISSN 121–128

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