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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 2 > Etsuko Fujii1), Atsuhiko Kato1), Yu Jau Chen2), Koichi Matsubara2,3), Yasuyuki Ohnishi4), and Masami Suzuki1)

The status of donor cancer tissues affects the fate of patient-derived colorectal cancer xenografts in NOG mice

Etsuko Fujii1), Atsuhiko Kato1), Yu Jau Chen2), Koichi Matsubara2,3), Yasuyuki Ohnishi4), and Masami Suzuki1)
1)Research Division, Chugai Pharmaceutical Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan 2)Chugai Pharmabody Research, Pte. Ltd., 3 Biopolis Drive, #04-11 Synapse, Singapore 138623 3)PharmaLogicals Research, Pte. Ltd., 11 Biopolis Way, #05-08/09 Helios, Singapore 138667 4)Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821 Japan
Abstract :

Patient-derived xenografts (PDXs) of tumors are increasingly becoming important tools for translational research in oncology. The NOD.Cg-Prkdcscid Il2rgtm1Sug/Jic (NOG) mouse is an efficient host for PDXs. Thus as a basis for future development of methods to obtain PDXs from various disease types, we have studied the factors that affect the outcome of transplantation of human colorectal cancer in NOG mice. Of the original donor cases examined, 73% had successful engraftment. The outcome of donor-matched tissues was consistent in most cases, and was thought to show that the condition of the host did not affect engraftment. Next we analyzed the tumor aggressiveness in terms of histology grade of the original tumor and found that they were related to engraftment. Detailed histopathological examination of the transplanted tissues strongly indicated that lymphocytes engrafted with the tumor cells affect engraftment. As a factor related to transplantation of lymphocytes, we studied the human IgG concentration in the serum of tumor-bearing mice, but there was no tendency for higher concentrations to result in unsuccessful engraftment. Finally, we studied the type, density and location of T cells in the original donor tissue to determine the immune contexture and found that the unsuccessful engraftment cases tended to have an adequate or coordinated immune contexture compared to successful engraftment cases. From these results, we concluded that the aggressiveness and the T cell infiltration of the original tumor affect the outcome of transplantation in the NOG mouse

Keywords :
immunoglobulin, NOG mouse, patient-derived xenograft, T lymphocyte, tumor aggressiveness

Date Deposited : 06 Jan 2016 11:39

Last Modified : 06 Jan 2016 11:39

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/2/_contents

Volume 64, Number 2, - 2015 , ISSN 181–190

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