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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 2 > Akira ISHIKAWA1), Makoto SUGIYAMA2,3), Eiichi HONDO2), Keiji KINOSHITA1), and Yuki YAMAGISHI1)

Development of a novel pink-eyed dilution mouse model showing progressive darkening of the eyes and coat hair with aging

Akira ISHIKAWA1), Makoto SUGIYAMA2,3), Eiichi HONDO2), Keiji KINOSHITA1), and Yuki YAMAGISHI1)
1)Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan 2)Laboratory of Animal Morphology and Function, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan 3)Present address: Laboratory of Veterinary Anatomy, School of Veterinary Medicine, Kitasato University, Higashi, Towada, Aomori 034-8628, Japan
Abstract :

Oca2p-cas (oculocutaneous albinism II; pink-eyed dilution castaneus) is a coat color mutant gene on mouse chromosome 7 that arose spontaneously in wild Mus musculus castaneus mice. Mice homozygous for Oca2p-cas usually exhibit pink eyes and gray coat hair on the non-agouti genetic background, and this ordinary phenotype remains unchanged throughout life. During breeding of a mixed strain carrying this gene on the C57BL/6J background, we discovered a novel spontaneous mutation that causes darkening of the eyes and coat hair with aging. In this study, we developed a novel mouse model showing this unique phenotype. Gross observations revealed that the pink eyes and gray coat hair of the novel mutant young mice became progressively darker in color by approximately 3 months after birth. Light and transmission-electron microscopic observations revealed a marked increase in melanin pigmentation of coat hair shafts and choroid of the eye in the novel mice compared to that in the ordinary mice. Sequence analysis of Oca2p-cas revealed a 4.1-kb deletion involving exons 15 and 16 of its wild-type gene. However, there was no sequence difference between the two types of mutant mice. Mating experiments suggested that the novel mutant phenotype was not inherited in a simple fashion, due to incomplete penetrance. The novel spontaneous mutant mouse is the first example of progressive hair darkening animals and is an essential animal model for understanding of the regulation mechanisms of melanin biosynthesis with aging.

Keywords :
animal model, coat color, hair darkening, mouse, Oca2

Date Deposited : 06 Jan 2016 11:43

Last Modified : 06 Jan 2016 11:43

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/2/_contents

Volume 64, Number 2, - 2015 , ISSN 207–220

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