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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 3 > Sari Suzuki1, 2), Masashi Ishikawa2), Takuya Ueda2), Yasuhiro Ohshiba1, 3), Yuki Miyasaka1, 3), Kazuhiro Okumura1, 4), Michinari Yokohama2), Choji Taya5), Kunie Matsuoka1), and Yoshiaki Kikkawa1, 3)

Quantitative trait loci on chromosome 5 for susceptibility to frequency-specific effects on hearing in DBA/2J mice

Sari Suzuki1, 2), Masashi Ishikawa2), Takuya Ueda2), Yasuhiro Ohshiba1, 3), Yuki Miyasaka1, 3), Kazuhiro Okumura1, 4), Michinari Yokohama2), Choji Taya5), Kunie Matsuoka1), and Yoshiaki Kikkawa1, 3)
1)Mammalian Genetics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan 2)Department of Bioproduction, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan 3)Graduate School of Medical and Dental Sciences, Niigata University, 1–757 Asahimachi, Niigata 951-8510, Japan 4)Laboratory of Experimental Animals, Chiba Cancer Center Research Institute, 666-2 Nitona-cho, Chuo-ku, Chiba 260-8717, Japan 5)Laboratory for Transgenic Technology, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan
Abstract :

The DBA/2J strain is a model for early-onset, progressive hearing loss in humans, as confirmed in the present study. DBA/2J mice showed progression of hearing loss to low-frequency sounds from ultrasonic-frequency sounds and profound hearing loss at all frequencies before 7 months of age. It is known that the early-onset hearing loss of DBA/2J mice is caused by affects in the ahl (Cdh23ahl) and ahl8 (Fscn2ahl8) alleles of the cadherin 23 and fascin 2 genes, respectively. Although the strong contributions of the Fscn2ahl8 allele were detected in hearing loss at 8- and 16- kHz stimuli with LOD scores of 5.02 at 8 kHz and 8.84 at 16 kHz, hearing loss effects were also demonstrated for three new quantitative trait loci (QTLs) for the intervals of 50.3–54.5, 64.6–119.9, and 119.9–137.0 Mb, respectively, on chromosome 5, with significant LOD scores of 2.80–3.91 for specific high-frequency hearing loss at 16 kHz by quantitative trait loci linkage mapping using a (DBA/2J × C57BL/6J) F1 × DBA/2J backcross mice. Moreover, we showed that the contribution of Fscn2ahl8 to early-onset hearing loss with 32-kHz stimuli is extremely low and raised the possibility of effects from the Cdh23ahl allele and another dominant quantitative trait locus (loci) for hearing loss at this ultrasonic frequency. Therefore, our results suggested that frequency-specific QTLs control early-onset hearing loss in DBA/2J mice.

Keywords :
ahl locus, chromosome 5, DBA/2J, early-onset hearing loss, quantitative trait loci (QTL)

Date Deposited : 07 Jan 2016 10:23

Last Modified : 07 Jan 2016 10:23

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/3/_contents

Volume 64, Number 3, March 2015 , ISSN 241–251

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